![]() ![]() FIB, SEM, TEM and fluorescence images used for 3D correlation are available on the BioImage Archive (S-BSST730, S-BSST729). FIB-SEM volume imaging data for the Sum159 cells and Chlamydomonas reinhardtii are deposited in EMPIAR under accession codes EMPIAR-10847, EMPIAR-10870. New 3DCT functions described in this work, scripts for cryo-FLM virtual slice series creation, 3D point-based registration and transformation of cryo-FLM data with respect to cryo-FIB-SEM data, and bUnwarpJ-based analysis of FIB images before and after milling are available on. The Python 3-ported 3DCT is available on. A Python script for post-processing of cryo-FIB-SEM volume imaging data as described in the method section is available in the SerialFIB GitHub repository. The documentation is available on GitHub ( ). A comprehensive video tutorial session is uploaded to YouTube. SerialFIB and a written tutorial can be obtained on: ( copy archived at swh:1:rev:0eaaaf66afa2d803440cea18af85c444df10478f). GUID: 9500E2E7-8F2B-423F-88AC-6EACED292026 Data Availability StatementĪll code developed in this work is available on GitHub. Cryogenic 3D-CLEM targeted lamella preparation of HeLa cells. Klumpe S, Fung HKH, Goetz SK, Plitzko JM, Mahamid J.Cryo-correlative fluorescence and FIB-SEM volume imaging of HeLa cells. Electron Microscopy Public Image Archive. Cryo-FIB-SEM data on Chlamydomonas reinhardtii cells. Cryo-FIB-SEM volume in a Sum159 human cell line. melanogaster ribosomes from tomograms collected on a cryo-lift-out lamella. Cryo-electron tomogram from a cryo-FIB lift-out lamella from Drosophilia melanogaster egg chamber. ![]() Cryo-electron tomogram of a 3D correlated lipid droplet in a cryo-FIB-milled HeLa cell. ![]() Cryo-electron tomogram of a cryo-FIB lamella of Emiliania huxleyi cells. Cryo-electron tomogram of a cryo-FIB lamella of a HeLa cell. Cryo-electron tomograms from cryo-FIB-lamellae of Sum159 human cell line prepared after cryo-FIB-SEM volume imaging. Subtomogram average of 80S ribosomes from a cryo-FIB-lamella of Sum159 human cell line prepared after cryo-FIB-SEM volume imaging. Cryo-electron tomograms from cryo-FIB-lamellae of Sum159 human cell line. Subtomogram average of 80S ribosomes from a cryo-FIB-lamella of Sum159 human cell line. ![]()
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